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Supercritical Fluid Extraction and Insecticidal Activity of Lamiaceae Plants
Sajfrtová, Marie ; Pavela, R. ; Karban, Jindřich ; Sovová, Helena
Natural insecticides based on bioactive extracts from plants represent a new trend in plant protection. Supercritical extraction (SFE) using CO2 is a modern extraction technique able to isolate high-added value compounds from plant without any thermal degradation, which allows to obtain extracts with high biological activity. In this work, insecticidal activity of SFE extracts from plant of mint family (Lamiaceae) has been studied and compared to activity of isolates obtained by conventional extraction techniques (hydrodistillation, organic solvent extraction). As a result of variation in extraction pressure, three types of extract were obtained: extract rich in volatile compounds (at 12 MPa), extract rich in oleoresins (at 28 MPa) and in polar compounds (5% of acetone used as modifier). Chemical composition and biological activity (LD50, antifeedant activity) of all isolates were evaluated and compared.
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Insekticidní aktivita rostlinných extraktů izolovaných různými metodami
Sajfrtová, Marie ; Sovová, Helena ; Karban, Jindřich ; Pavela, R.
The aim of this study was to compare the insecticidal activity of extracts obtained by SFE and traditional extraction method. Three types of extracts were prepared using the benefit of variable solvent power of supercritical carbon dioxide under different experimental conditions. Composition and product activities of the CO2 extracts were compared with hydrodistillation (HD) and Soxhlet extraction. The composition of volatile compounds in the isolates was determined by gas chromatography, and the toxicity and antifeedant effects of the extracts on larvae of Colorado potato beetle were evaluated.
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